CytoGem-XAI:A Hypergraph Neural Network Framework for Genome-Scale Metabolic Modeling and Interpretable Analysis

Genome-scale metabolic models are essential for understanding cellular metabolism, yet existing deep learning approaches remain black boxes, and traditional flux balance analysis (FBA) cannot provide sample-specific predictions. To our knowledge, CytoGem-XAI is the first framework to combine hypergraph neural network representation with interpretable, FBA-parallel analysis and sample-specific metabolic characterization. Built upon hypergraph representations where reactions are encoded as hyperedges connecting their participating metabolites, CytoGem-XAI introduces three analysis modules: perturbation-based carbon source importance ranking, hard intervention reaction bottleneck identification, and pathway-level topological attribution. Beyond prediction, CytoGem-XAI uniquely enables condition-dependent carbon source essentiality and reaction bottlenecks that vary with genetic background - capabilities absent from both traditional FBA and existing deep learning methods. Trained on 17,400 E.coli growth conditions using 10-fold cross-validation, our framework achieves 2 =0 .862,substantially outperforming AMN (R^2=0 .81,+6 .4%), FBA ( R^2=0 .62,+39%),and gradient boosting baselines (R^2 =0.71,+21%). Biological validation confirms that CytoGem-XAI identifies known essential carbon sources (e.g., alanine, malate) and rate-limiting enzymes (e.g., TCA cycle), while also revealing N-acetylmuramate - a peptidoglycan precursor - as a previously underappreciated essential nutrient.